Wastewater monitoring
wastewater as a diagnostic medium

PartnersResults of
the COVMON project
and results


Project implementation in 2024

1) In April 2024, an article was published: Sovová, K., Vašíčková, P., Valášek, V., Výravský, D., Očenášková, V., Juranová, E., Bušová, M., Tuček, M., Bencko, V., Zvěřinová Mlejnková, H.: SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters, Water Research X, Volume 23, 2024, 100220, ISSN 2589-9147,

Abstract: This study presents the results of systematic wastewater monitoring of SARS-CoV-2 RNA and basic wastewater parameters from four different wastewater treatment plants (WWTPs) in the Czech Republic over the 2020–2022 epidemic. Two-step reverse-transcription quantitative PCR targeting genes encoding the N and Nsp12 proteins was employed to detect SARS-CoV-2 RNA loading in 420 wastewater samples. The results obtained were used to evaluate the potential of wastewater analysis for describing the epidemiological situation in cities of different sizes and determining temporal differences based on the prevailing SARS-CoV-2 variant. Strong correlations between the number of active and hospitalised COVID-19 cases in each WWTP catchment area and the concentration of SARS-CoV-2 RNA detected in the wastewater clearly demonstrated the suitability of this wastewater-based epidemiological approach for WWTPs of different sizes and characteristics, despite differences in SARS-CoV-2 variant waves, with some WWTPs showing high predictive potential. This study demonstrated on the data from the Czech Republic that targeted systematic monitoring of wastewater provides sufficiently robust data for surveillance of viral loads in sample populations, and thus contributes to preventing the spread of infection and subsequent introduction of appropriate measures.

Keywords: Wastewater-based epidemiology; COVID-19; Virus; PCR; Wastewater treatment plant

2) Orientation determination of SARS-CoV-2 RNA is performed once a month at the influent of the Brno WWTP – see graph

3) Lecture for the Society for Effective Altruism: Biosafety & data: early response to pandemics through wastewater monitoring.

4) Updating of the VÚV website (HEIS): here

5) Updating the website of the European Commission:

Project implementation in 2023

Last update: 29 April 2024


T. G. Masaryk Water Research Institute, p.r.i.
Podbabská 2582/30
160 00 Praha 6
Czech Republic

Project leader

RNDr. Hana Zvěřinová Mlejnková, Ph.D.
+420 220 197 376
+420 724 583 662

Technically provides

TGM WRI Hydroecological
Information System
© Copyright: T. G. Masaryk Water Research Institute, public research institution. Design: Jiří Picek. 2022. Photo: members of project team, unless stated otherwise. Visits of pages